Human Systems Directorate
DoD Office of HSD Animal Protections Programs
Our goal is to ensure DoD research is conducted ethically and in compliance with DoD Instruction 3216.01, Use of Animals in DoD Programs
Vision:
Department of Defense research and training with animals adheres to the highest standards in the world, supported by a culture of care and compliance that provides animals with humane, compassionate care.

Mission:
Every institution within the Department of Defense is structured to ensure a culture of compliance with federal laws, and identifies best practices, carefully balancing the benifits of animal use with the impact that each study will have on the animals.
DoD Guidance Documents
- DoD Instruction Implementing the Animal Welfare Act and Regulations: DoD Instruction 3216.01 “Use of Animals in DoD Conducted and Supported Research and Training”
- DoD Component Policy Implementing DoD Instruction 3216.01: DHA-MSR 6025.02 “The Care and Use of Animals in DoD Research, Development, Test, and Evaluation (RDT&E) or Training Programs”
- DoD Standard Animal Use Protocol Format. DoD organizations must use this protocol format when proposing to use animals covered under DoD Instruction 3216.01. Non-DoD organizations being sponsored by DoD shall either use the DoD Standard Protocol Format or provide all pertinent information contained in the DoD Standard Protocol.
- DoD Semi-Annual Program/Facility Inspection Checklist (DD Form 2856)
Reference Documents
- Animal Welfare Act and Regulations
- Guide for the Care and Use of Laboratory Animals
- USDA Animal Care Policy Manual
- Public Health Service Policy on Humane Care and Use of Laboratory Animals
- The Interagency Coordinating Committee on the Validation of Alternative Methods to establish, wherever feasible, guidelines, recommendations, and regulations that promote the regulatory acceptance of new or revised scientifically valid toxicological tests that protect human and animal health and the environment while reducing, refining or replacing animal tests and ensuring human safety and product effectiveness.